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ORIGINAL ARTICLE
Year : 2017  |  Volume : 2  |  Issue : 2  |  Page : 137-142

Evaluation of glucose-6-phosphate dehydrogenase enzyme deficiency and methemoglobin concentration in blood donors in a Nigerian Tertiary hospital-based blood bank


1 Department of Haematology, College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Anambra State, Nigeria
2 Department of Medical Laboratory Science, College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Anambra State, Nigeria

Correspondence Address:
John C Aneke
Department of Haematology, College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Anambra State
Nigeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/GJTM.GJTM_21_17

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Background: Glucose-6-phosphate dehydrogenase (G6PD) enzyme deficiency and methemoglobinemia adversely impact on blood transfusion safety by significantly increasing blood storage lesion. Objective: To determine G6PD enzyme deficiency and methemoglobin levels among blood donors in a tertiary hospital-based blood bank in Nigeria. Subjects and Methods: One hundred blood donors who met the criteria for blood donation were prospectively studied. Two milliliters of venous blood was collected from each participant into potassium-ethylenediaminetetraacetic acid specimen containers and analyzed for G6PD status and methemoglobin levels by spectrophotometry, on the same day of sample collection. Results: Among the donors, 43% had normal G6PD activity (9.32 ± 2.26 U/gHb), 44% had partially enzyme deficiency (4.92 ± 1.33 U/gHb), while 13% had total deficiency (0.47 ± 3.49 U/gHb); these were statistically different (P < 0.001). Methemoglobin concentration was elevated in 25% of study participants (3.05 ± 2.30%), while it was normal in 75% (0.99% ± 0.60%); these differences were statistically different (P < 0.001). Conclusion: A significant proportion of our blood donor set has G6PD enzyme deficiency (partial or total) as well as evidence of oxidation of hemoglobin; these findings have adverse implications on transfusion safety.


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