|Year : 2022 | Volume
| Issue : 1 | Page : 7-11
Therapeutic thrombocytapheresis as an important tool in the management of symptomatic hyperthrombocytosis: A single-institution experience from India
Sudipta Sekhar Das1, Rathindra Nath Biswas1, Subrata Sen1, Anupam Chakrapani2
1 Department of Transfusion Medicine, Apollo Gleneagles Hospitals, Kolkata, West Bengal, India
2 Department of Clinical Hematology, Apollo Gleneagles Hospitals, Kolkata, West Bengal, India
|Date of Submission||18-Jun-2021|
|Date of Decision||22-Oct-2021|
|Date of Acceptance||02-Nov-2021|
|Date of Web Publication||29-Apr-2022|
Dr. Sudipta Sekhar Das
Department of Transfusion Medicine, Apollo Gleneagles Hospitals, Kolkata, West Bengal
Source of Support: None, Conflict of Interest: None
Background & Objectives: Hyperthrombocytosis may cause acquired thrombosis-related symptoms and fatal vascular complications. Currently, therapeutic platelet reduction (TPR) with medical therapy remains the mainstay of hyperthrombocytosis management. We encounter patients with high platelet counts with requests for TPR. Here, we share our experience of TPR procedures in patients with symptomatic hyperthrombocytosis due to various underlying etiologies. Methods: The study from January 2013 to October 2020 included 36 patients of hyperthrombocytosis who underwent 82 TPR procedures by apheresis technology. Patient details were obtained from the treatment file and all procedures were performed following recommended instructions and protocol. Statistical analysis was done using the SPSS statistical package. Results: The median age of patients was 53 years with mean hemoglobin, platelet count, plateletcrit, and platelet distribution width of 9.9 g/dL, 1711.3 × 106/mL, 0.57%, and 43.4%, respectively. A total of 26 patients had primary thrombocytosis. The mean TPR procedure time, whole blood volume processed, and anticoagulant used were 162 min, 5070 mL, and 430.3 mL, respectively. The mean reduction of platelets in patients who underwent two and three procedures was 72.4% and 82.7%, respectively (P = 0.003). Conclusion: We conclude that TPR is a useful method in reducing platelet count rapidly in hyperthrombocytosis. It relieves patients of acute symptoms and prevents thrombotic events. The decision to perform TPR should be individualized and based on the clinical scenario, degree of thrombocytosis, and risk factors associated with TPR procedures.
Keywords: Apheresis, essential thrombocythemia, hyperthrombocytosis, therapeutic platelet reduction, thrombocytapheresis
|How to cite this article:|
Das SS, Biswas RN, Sen S, Chakrapani A. Therapeutic thrombocytapheresis as an important tool in the management of symptomatic hyperthrombocytosis: A single-institution experience from India. Glob J Transfus Med 2022;7:7-11
|How to cite this URL:|
Das SS, Biswas RN, Sen S, Chakrapani A. Therapeutic thrombocytapheresis as an important tool in the management of symptomatic hyperthrombocytosis: A single-institution experience from India. Glob J Transfus Med [serial online] 2022 [cited 2022 Dec 1];7:7-11. Available from: https://www.gjtmonline.com/text.asp?2022/7/1/7/344337
| Introduction|| |
Hyperthrombocytosis is defined as platelet counts >800–1000 × 109/L.,,, and may be an incidental finding in patients or may be present with thrombocytosis-related symptoms and complications. Conditions such as benign myeloproliferative neoplasm (MPN) such as essential thrombocythemia (ET), chronic myeloproliferative diseases (CMPD) such as chronic myeloid leukemia (CML), malignancy, chronic inflammatory diseases, bone marrow recovery following myelosuppressive therapy, splenectomy, infections, trauma, hemorrhage, or burns may cause hyperthrombocytosis.,, While hyperthrombocytosis is reported in up to 50% of MPN patients, its incidence is lower in CML., Symptoms are predominantly vasomotor and may include headaches, dizziness, blurring of vision, syncope, and erythromelalgia. In addition, patients with hyperthrombocytosis are at increased risk for major thrombotic and hemorrhagic complications., Approximately 90% of patients with ET show a mutually exclusive JAK2, CALR, or myeloproliferative leukemia (MPL) mutation. Patients with hyperthrombocytosis who have active ongoing complications or have additional cardiovascular and/or thrombotic risk factors need treatment. While long term management includes drugs like hydroxyurea, anagrelide or interferon-alfa; therapeutic thrombocytapheresis or cytoreduction or therapeutic platelet reduction (TPR) is reserved for acute events. However treatment of uncomplicated hyperthrombocytosis is still a subject of debate. The choice and intensity of cytoreduction depends on the severity of thrombocytosis and impending risk for ischemic/hemorrhagic events.
TPR is currently a Category II (second-line therapy) American Society for Apheresis (ASFA) recommendation in symptomatic primary thrombocytosis and Category III (optimum role not established, decision individualized) in the prophylactic high-risk ET or secondary thrombocytosis setting. The role of TPR as a prophylactic measure to prevent untoward consequences in patients at high risk for occlusive or hemorrhagic events has also been described., Ours being a tertiary care hospital with a dedicated blood center with apheresis facilities, we encounter patients with high platelet counts with requests for TPR.
Aims and objectives
Here, we share our experience of TPR procedures in patients with symptomatic hyperthrombocytosis due to various underlying etiologies.
| Materials and Methods|| |
The observational prospective study from January 2013 to October 2020 included 36 patients of hyperthrombocytosis who underwent 82 TPR procedures. All procedures were performed by the same apheresis team following the manufacturer's instructions and departmental standard operating procedure (SOP).
Ethical committee clearance
Due ethical clearance was obtained from the hospital ethics committee to conduct the study. Procedures were done using recommended apheresis equipment (Amicus version 3.21, Fresenius Kabi AG, Bad Homburg, Germany), closed system disposable kits, and anticoagulants (acid-citrate-dextrose-A [ACD-A]) after obtaining prescription from the treating physician. Before starting TPR, detailed demographic, laboratory, and clinical details were obtained from each patient and documented accordingly. For patients with poor antecubital venous access, indwelling central or peripheral venous catheters were used for the procedure. The of each procedure was based on the maximum volume or number of platelets that could be removed based on patient weight, height, hematological values, and total blood volume. As per SOP, a maximum of 1.5 times blood volume was processed over any range of platelet counts.
Procedure details such as total blood volume processed, ACD-A volume infused, procedure time, and blood flow rate were recorded for each procedure in the procedure register. All patients were administered prophylactic oral or intravenous calcium (1000 mg) 30–45 min before procedure to prevent citrate-related toxicities. All were explained the details of procedure before starting it. They were advised to report discomfort, if any, to the apheresis team during or after the procedure and were asked to take adequate rest after the procedure. Adverse reactions, if any, were managed appropriately and documented following departmental SOP.
Statistical analysis was done using the SPSS statistical package (IBM, 2015, Armonk, New York, USA). Demographic, laboratory, and clinical details of patients' were obtained from hospital computer software, captured in the MS office excel sheet, and statistically analyzed. Quantitative variables were calculated as mean ± standard deviation or N (%) and analyzed using the paired Student's t-test. “P” value of < 0.05 was considered statistically significant.
| Results|| |
The observational prospective study included 36 patients with hyperthrombocytosis who underwent therapeutic thrombocytapheresis for platelet reduction. The median age of patients was 53 years with a male preponderance. [Table 1] describes the demographic, laboratory, and clinical features of these patients. The mean hemoglobin, platelet count, plateletcrit, and platelet distribution width were observed to be 9.9 g/dL, 1711.3 × 106/mL, 0.57%, and 43.4% respectively. A total of 26 patients (72.2%) had primary thrombocytosis mainly caused by ET and CML. Out of 10 cases of secondary thrombocytosis, 5 (50%) had hyperthrombocytosis following surgery. Thirty-one (86.1%) patients complained of constant lightheadedness or dizziness, followed by 29 (80.6%) with persistent headache. Tingling sensation in extremities was observed in 21 (58.3%) patients. A total of 82 therapeutic thrombocytapheresis procedures were performed in 36 patients using the Amicus apheresis equipment [Table 2]. Forty-nine (60.5%) procedures were done using central or peripheral catheters under aseptic and antiseptic measures. Mean procedure time, whole blood volume processed, and anticoagulant used were 162 min, 5070 mL, and 430.3 mL, respectively. Mean platelet collection efficiency of the equipment was normal and calculated to be 64.7%. The mean postprocedure platelet count in the patients was observed to be 383.4 × 106/mL.
|Table 1: Demographic, laboratory, and clinical features of patients undergoing therapeutic platelet reduction (n=36)|
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[Figure 1] shows the gradual decrement of platelet counts in patients who underwent 2 or 3 procedures of therapeutic thrombocytapheresis consecutively. The mean preprocedure platelet counts were observed to be 2200.1 × 106/mL (range: 1661.3–2921.3) and 1401.9 × 106/mL (range: 1120.2–1648.4) in patients undergoing three and two procedures, respectively (P = 0.039). In patients who underwent three procedures, the mean platelet count reduced from 2200.1 × 106/mL to 379.6 × 106/mL (82.7%) after the third procedure. The mean reduction of platelets in patients who underwent two procedures was 72.4% (P = 0.003). We observed that the mean reduction of platelets after the first procedure was significantly high when the mean preprocedure platelet count was more (2200.1 × 106/mL vs. 1401.9 × 106/mL). The mean reduction of platelet count was 51.3% after the first procedure in patients undergoing three procedures; the reduction was 42.6% in those who underwent two procedures (P = 0.032). Reduction of platelets in subsequent procedures was comparable in both categories of patients.
|Figure 1: Decrement of platelet counts in patients undergoing therapeutic platelet reduction (N=36)|
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| Discussion|| |
All patients of hyperthrombocytosis need evaluation to differentiate a primary proliferative process or a reactive response to an inciting condition as the cause of elevated platelet counts. The present study observed 26 patients of primary thrombocytosis and 10 patients of secondary thrombocytosis. While CML was the major cause of primary thrombocytosis, the main inciting condition leading to secondary thrombocytosis was postsurgical events. It was interesting to note that all 10 patients requiring three TPR procedures for cytoreduction belonged to the category of primary thrombocytosis. Considering all patients of primary thrombocytosis, the mean preprocedure platelet count was observed to be 1507.9 × 106/mL. A total of 18 of these patients (69.2%) revealed JAK2 mutations and one showed MPL mutations (exons 10 and 11) who was otherwise negative for JAK2 mutations. In an Indian study by Sazawal et al., JAK2 V617F mutation was found in 68% of CMPD, 82% of polycythemia vera (PV), and 70% of ET. The median age at diagnosis of ET is in the sixth decade of life, with <20% of patients being diagnosed below 40 years. The median age of patients under the present study was 53 years and that with ET only was 59 years. As high as 25 (69.4%) patients were males with an M: F ratio of 2.3:1. Ten patients who underwent three consecutive TPR procedures had a mean preprocedure platelet count of 2200.1 × 106/mL, which was significantly higher than those who underwent two TPR procedures (P = 0.039).
The risk of thrombotic complications with reactive thrombocytosis is low, and 1.6% of patients with reactive thrombocytosis had thrombotic complications in one large case series. In clonal thrombocytosis, especially in ET and PV, thrombotic complications are a major cause of morbidity and mortality and the primary factor in determining treatment strategy. We observed thrombotic complications in 7 (63.6%) patients of ET in the form of stroke or transient ischemic attack.
Previous authors have suggested that aged patients (>60 years) with prior thrombohemorrhagic event and platelet counts >1500 × 106/mL confer a high risk for thrombohemorrhagic events in primary thrombocytosis and respond well to cytoreductive treatment. Selected patients of hyperthrombocytosis may need rapid platelet reduction during the first critical days. Such intervention provides symptomatic relief and prevents new or worsening major vascular complications. Cases illustrating the role of TPR to prevent untoward consequences in patients at high risk for occlusive or hemorrhagic events have also been described., We performed 82 procedures of TPR in 36 patients and successfully reduced the platelet burden in all patients.
A mean whole blood volume of 5070 mL was processed in a mean time of 162 min using 430.3 mL of mean anticoagulant volume. A total of 49 (59.8%) patients required central or peripheral catheterization due to poor peripheral venous access. Methods involving TPR in patients and plateletpheresis in normal donors are almost identical. TPR is performed on sick individuals and involves the processing of maximum allowable whole blood volume to reduce platelets optimally and control the state of hyperthrombocytosis. As procedure time directly concerns the donor/patient in apheresis, it is an important parameter to evaluate their safety and comfort. While Burgstaler et al. and Das et al. observed mean procedure time of 78 min and 79.6 min by Amicus during normal plateletphersis, other studies on TPR estimated procedure time between 103 min and 218 min using various apheresis equipments.,,,
Apheresis is a safe procedure without significant complications, but at times, symptoms due to citrate toxicity and other adverse events may cause discomfort to the donors. Adverse events such as citrate toxicity and vasovagal reactions were observed in four patients (4.9%). Despite administering prophylactic calcium (1000 mg) as suggested by previous authors, citrate toxicity was found in two female patients of primary thromboctyosis., Various reasons of citrate toxicity despite prophylactic calcium administration have been observed and discussed by previous authors.,
Plateletpheresis has effects on coagulation parameters. Decrease in prothrombotic FVIII, fibrinogen, antithrombin, protein C, and protein S levels has been observed by previous authors., Due to limited resources, only FVIII and fibrinogen were measured in patients under study. Although FVIII and fibrinogen values reduced from 1.08 IU/mL and 325.3 mg/dL to 1.02 IU/mL and 298.7 mg/dL, respectively, at every instance, the values were found to be within recommended ranges.
The usefulness of rapid plateletpheresis in preventing additional morbidity in patients with thrombocytosis by removing large numbers of circulating platelets has already been documented., While Taft et al. were able to collect 16–26 × 1011 platelets, and up to 98 × 1011 when processing volumes of more than 10 L by using the Haemonetics model 10 and the Aminco Celltrifuge, McCarthy et al. found a reduction between 1580 and 2665 × 103/μl of peripheral platelets by apheresis when using the CS-3000 Fenwal separator., Das et al. found the Haemonetics MCS + to be highly efficient in the acute management of ET, and consecutive three procedures could reduce the platelet count significantly and prepare the patient for cardiac surgery. After successful completion of cytoreduction therapy using apheresis technology, the mean postprocedure platelet count in the current study was observed to be 383.4 × 106/mL with a significant mean decrement of 76.4% (P = 0.027).
Mean platelet count reduced by 82.7% and 72.4%, respectively, in patients who underwent three and two procedures. We also observed that the mean reduction of platelets after the first procedure was statistically significant (P = 0.032) when the mean preprocedure platelet count was higher. Such a result may be expected because, with a higher number of platelets in the patient/donor, a greater platelet yield is obtained in the end product, thereby causing a greater fall in the person's platelets after TPR/plateletpheresis.,
Data on the clinical utility of TPR are largely based on anecdotal experience, and the current AFSA guidelines are not supported by randomized controlled trials. TPR should be considered with relative urgency in patients with platelet counts above 1500 × 106/mL and an increased risk of major hemorrhage, especially in settings where cytoreductive agents are contraindicated or less desirable due to their relatively slow onset of action.
| Conclusion|| |
TPR is a useful method in reducing platelet count rapidly in hyperthrombocytosis and relieving patients of acute symptoms and preventing them from thrombotic events. Such procedures should always be performed very meticulously to maintain patient safety. Moreover, decision to perform TPR should be individualized and based on the clinical scenario, degree of thrombocytosis, and risk factors associated with TPR procedures.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Dalai BI, Nyokong AM. Hyperthrombocytosis: A Clinicohematological study. Lab Med 1992;23:811-4.
Preston FE. Essential thrombocythaemia. Lancet 1982;1:1021.
Schafer AI. Thrombocytosis and thrombocythemia. Blood Rev 2001;15:159-66.
Mitus AJ, Schafer AI. Thrombocytosis and thrombocythemia. Hematol Oncol Clin North Am 1990;4:157-78.
Hehlmann R, Jahn M, Baumann B, Köpcke W. Essential thrombocythemia. Clinical characteristics and course of 61 cases. Cancer 1988;61:2487-96.
Robbins G, Barnard DL. Thrombocytosis and microthrombocytosis: A clinical evaluation of 372 cases. Acta Haematol 1983;70:175-82.
Finazzi G, Carobbio A, Thiele J, Passamonti F, Rumi E, Ruggeri M, et al
. Incidence and risk factors for bleeding in 1104 patients with essential thrombocythemia or prefibrotic myelofibrosis diagnosed according to the 2008 WHO criteria. Leukemia 2012;26:716-9.
Sora F, Iurlo A, Sica S, Latagliata R, Annunziata M, Galimberti S, et al
. Chronic myeloid leukaemia with extreme thrombocytosis at presentation: Incidence, clinical findings and outcome. Br J Haematol 2018;181:267-70.
Tefferi A, Fonseca R, Pereira DL, Hoagland HC. A long-term retrospective study of young women with essential thrombocythemia. Mayo Clin Proc 2001;76:22-8.
Chistolini A, Mazzucconi MG, Ferrari A, la Verde G, Ferrazza G, Dragoni F, et al
. Essential thrombocythemia: A retrospective study on the clinical course of 100 patients. Haematologica 1990;75:537-40.
Tefferi A, Guglielmelli P, Larson DR, Finke C, Wassie EA, Pieri L, et al
. Long-term survival and blast transformation in molecularly annotated essential thrombocythemia, polycythemia vera, and myelofibrosis. Blood 2014;124:2507-13; quiz 2615.
Barbui T, Tefferi A, Vannucchi AM, Passamonti F, Silver RT, Hoffman R, et al
. Philadelphia chromosome-negative classical myeloproliferative neoplasms: Revised management recommendations from European LeukemiaNet. Leukemia 2018;32:1057-69.
Tefferi A. Recent progress in the pathogenesis and management of essential thrombocythemia. Leuk Res 2001;25:369-77.
Schafer AI. Thrombocytosis: When is an incidental finding serious? Cleve Clin J Med 2006;73:767-74.
Schwartz J, Winters JL, Padmanabhan A, Balogun RA, Delaney M, Linenberger ML, et al
. Guidelines on the use of therapeutic apheresis in clinical practice-evidence-based approach from the writing committee of the American society for apheresis: The sixth special issue. J Clin Apher 2013;28:145-284.
Das SS, Bose S, Chatterjee S, Parida AK, Pradhan SK. Thrombocytapheresis: Managing essential thrombocythemia in a surgical patient. Ann Thorac Surg 2011;92:e5-6.
Larison J, Fatteh MM. Plateletpheresis as a preventive measure to complications of asymptomatic cases of idiopathic thrombocythemia. J Med Assoc Ga 1978;67:296-7.
Schafer AI. Thrombocytosis. JAMA 2015;314:1171-2.
Sazawal S, Bajaj J, Chikkara S, Jain S, Bhargava R, Mahapatra M, et al
. Prevalence of JAK2 V617F mutation in Indian patients with chronic myeloproliferative disorders. Indian J Med Res 2010;132:423-7.
] [Full text]
Jensen MK, de Nully Brown P, Nielsen OJ, Hasselbalch HC. Incidence, clinical features and outcome of essential thrombocythemia in a well-defined geographical area. Eur J Haematol 2000;65:132-9.
Griesshammer M, Bangerter M, Sauer T, Wennauer R, Bergmann L, Heimpel H. Aetiology and clinical significance of thrombocytosis: Analysis of 732 patients with an elevated platelet count. J Intern Med 1999;245:295-300.
Bleeker JS, Hogan WJ. Thrombocytosis: Diagnostic evaluation, thrombotic risk stratification, and risk-based management strategies. Thrombosis 2011;2011:536062.
Vannucchi AM, Barbui T, Cervantes F, Harrison C, Kiladjian JJ, Kröger N, et al
. Philadelphia chromosome-negative chronic myeloproliferative neoplasms: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol 2015;26:v85-99.
Goldfinger D, Thompson R, Lowe C, Kurz L, Belkin G. Long-term plateletpheresis in the management of primary thrombocytosis. Transfusion 1979;19:336-8.
Burgstaler EA, Winters JL, Pineda AA. Paired comparison of Gambro Trima Accel versus Baxter Amicus single-needle plateletpheresis. Transfusion 2004;44:1612-20.
Das SS, Sen S, Zaman RU, Biswas RN. Plateletpheresis in the era of automation: Optimizing donor safety and product quality using modern apheresis instruments. Indian J Hematol Blood Transfus 2021;37:134-9.
Das SS, Bhattacharya S, Sen S. Managing uncontrolled postsplenectomy reactive thrombocytosis in Idiopathic thrombocytopenic purpura: Role of thrombocytapheresis. Transfus Apher Sci 2013;49:171-3.
Singh A, Nityanand S, Sonker A, Kumar S. Successful use of the cell separator hemonetics multicomponent collection system+for therapeutic thrombocytapheresis in a low body weight child of essential thrombocythemia. Asian J Transfus Sci 2015;9:207-9.
] [Full text]
Dogra K, Fulzele P, Rout D, Chaurasia R, Coshic P, Chatterjee K. Adverse events during apheresis procedures: Audit at a tertiary hospital. Indian J Hematol Blood Transfus 2017;33:106-8.
Bassi R, Thakur KK, Bhardwaj K. Plateletpheresis adverse events in relation to donor and plateletpheresis session profile. Iraqi J Hematol 2017;6:38-42. [Full text]
Yilmaz M, Dikmen T, Sonmez M, Akdogan E, Durmus A, Omay SB, et al
. Change of coagulation parameters after double plateletpheresis. Transfus Apher Sci 2007;37:161-3.
Siti Nadiah AK, Nor Asiah M, Nur Syimah AT, Normi M, Anza E, Aini AN, et al
. Effects of plateletpheresis on blood coagulation parameters in healthy donors at National Blood Centre, Kuala Lumpur, Malaysia. Transfus Apher Sci 2013;49:507-10.
Grima KM. Therapeutic apheresis in hematological and oncological diseases. J Clin Apheresis 2000;15:28-52.
Taft EG, Babcock RB, Scharfman WB, Tartaglia AP. Plateletpheresis in the management of thrombocytosis. Blood 1977;50:927-33.
McCarthy LJ, Graves VL, Eigen H, McGuire WA. Platelet apheresis for extreme thrombocytosis in an 11-year-old girl with CML. Transfus Med 1991;1:187-9.
Landzo E, Berbić-Fazlagić J, Sofo-Hafizović A. The influence of initial values of donor platelets count on the efficiency of plateletpheresis with the cell separator Fenval Baxter Amicus s.v. 2.51. Med Arh 2009;63:67-70.
Chaudhary R, Das SS, Ojha S, Verma S, Khetan D. Comparative study of automated plateletpheresis using five different apheresis systems in a tertiary care hospital. Transfus Apher Sci 2009;40:99-103.
Boddu P, Falchi L, Hosing C, Newberry K, Bose P, Verstovsek S. The role of thrombocytapheresis in the contemporary management of hyperthrombocytosis in myeloproliferative neoplasms: A case-based review. Leuk Res 2017;58:14-22.
[Table 1], [Table 2]