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  Indian J Med Microbiol
 

Figure 4: DBS-0 like allele in cis to DAR2.00 allele. BAGene Partial D-TYPE gel view showed lanes 1-10 for RHD exons 1-10, respectively. Lane M is FastRuler™ LowRange DNA Ladder. This showed amplification of exon 1 (134 bp), 2 (146 bp), 3 (118 bp), 4 (135 bp), 6 (132 bp), 7 (120 bp), exon 9 (184 bp), and exon 10 (132 bp). No amplification bands of exon 5 (132 bp) and intron 7/exon 8 (637 bp) in lanes 5 and 8 suggest DBS-0 and DAR2.00, respectively. Since no serological E antigen was detected [Figure 1], the case was considered DBS-0 like allele. A polymerase chain reaction positive control with human growth hormone that gives a 434 bp was run along each polymerase chain reaction. The D status was reclassified as D positive (Partial D)

Figure 4: <i>DBS-0</i> like allele in cis to <i>DAR2.00</i> allele. BAGene Partial D-TYPE gel view showed lanes 1-10 for <i>RHD</i> exons 1-10, respectively. Lane M is FastRuler™ LowRange DNA Ladder. This showed amplification of exon 1 (134 bp), 2 (146 bp), 3 (118 bp), 4 (135 bp), 6 (132 bp), 7 (120 bp), exon 9 (184 bp), and exon 10 (132 bp). No amplification bands of exon 5 (132 bp) and intron 7/exon 8 (637 bp) in lanes 5 and 8 suggest <i>DBS-0</i> and <i>DAR2.00,</i> respectively. Since no serological E antigen was detected [Figure 1], the case was considered <i>DBS-0</i> like allele. A polymerase chain reaction positive control with human growth hormone that gives a 434 bp was run along each polymerase chain reaction. The D status was reclassified as D positive (Partial D)