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  Indian J Med Microbiol
 

Figure 6: Singleplex real-time polymerase chain reaction analysis for the detection of RHD exon 5. QuantiNova SYBR®Green real-time polymerase chain reaction was performed using Rotor-Gene Q. Both Rh-positive control and the sample 82 (case report sample) showed amplification of RHD exon 5 (a). To confirm the correct exon was amplified, DNA gel electrophoresis was performed and showed 157 bp amplification of the desired RHD exon 5 with Rh+ (Bu and NE) controls and sample 82 (b). Lane C is FastRuler™ Low Range DNA Ladder

Figure 6: Singleplex real-time polymerase chain reaction analysis for the detection of RHD exon 5. QuantiNova SYBR®Green real-time polymerase chain reaction was performed using Rotor-Gene Q. Both Rh-positive control and the sample 82 (case report sample) showed amplification of RHD exon 5 (a). To confirm the correct exon was amplified, DNA gel electrophoresis was performed and showed 157 bp amplification of the desired RHD exon 5 with Rh+ (Bu and NE) controls and sample 82 (b). Lane C is FastRuler™ Low Range DNA Ladder